At the age of twenty, after refusing opportunities to marry, she made a perpetual vow of chastity and took the third secular order of St. Francis of Assisi.

When her parents died, she moved to Valencia to live in a beguinage, where she practiced penance and prayer, and while doing so was said to be often thrown into "a state of ecstasy."

This was the case with Blessed Margarita Agulló, although her biographer took great care to hide them under the euphemism of "a red sign" on her chest or of the simple feeling, which was not palpable reality, of stigmata.

[1]Archbishop Juan de Ribera of Valencia, arranged to house Agullona near the Real College of Corpus Christi (next to the Patriarch Church).

[3][4] The first outcome of that work was titled Brief Relationship of Life, Virtues and Miracles of the humble Servant of Sister Margarita Agulló.