Marie-Denise Villers (née Lemoine; 1774 – 19 August 1821) was a French painter who specialized in portraits.

Two of her three sisters, Marie-Victoire Lemoine (1754–1820) and Marie-Élisabeth Gabiou (1755–1812), as well as distant cousin Jeanne-Elisabeth Chaudet (1767–1832), were all trained as portraitists.

It was in the Paris Salon of 1799 that she met the artist Anne-Louis Girodet-Trioson, and also began to take painting lessons with François Gérard and Jacques-Louis David.

Villers' most famous painting, Portrait of Charlotte du Val d'Ognes (1801) has been attributed to various artists and shown under a variety of titles through its long history.

Originally, the portrait was in the du Val d'Ognes family for generations, where it had been attributed to Jacques-Louis David.