Mario Chamlee (May 29, 1892 – November 13, 1966) was one of the lyric tenors who inherited several roles associated with Enrico Caruso at the Metropolitan Opera.

A year later, Chamlee went on tour with the Aborn Opera Company as "Mario Rodolfi", where he sang with soprano Ruth Miller.

During two and a half years of mandatory military service, during World War I, Chamlee served as a member of the Argonne Players, a group of army soldiers who sang and entertained troops on the front line.

One of Chamlee's earliest supporters, Gus Haenschen, who directed the popular-music records of the Brunswick company, stated in several interviews that Brunswick's classical-music director, Walter B. Rogers, worked with Chamlee to imitate Caruso's phrasing and dynamics as heard on his (Caruso's) Victor Red Seal recordings.

Although he was always well received by opera fans and critics alike across America and around the world and his records sold well, he never achieved the same level of recognition of his talents and abilities that his Italian contemporaries did, and Chamlee has been largely overlooked and forgotten in time.