[2][3] Marquette University High School is located at 3401 W. Wisconsin Avenue in the Merrill Park Neighborhood on Milwaukee's west side.

The Gordon Henke Center was completed in 1994 which included a new main entrance on W. Michigan St.

In 2008, the attached Jesuit residence was demolished to make way for a 1-story administrative and fine arts addition.

[4][5] The Hilltoppers' home field for soccer and track is Quad/Park, located a few blocks away adjacent to Merrill Park.

[6][7][8] Nicknamed the "Hilltoppers", MUHS teams compete in the Greater Metro Conference of the Wisconsin Interscholastic Athletic Association (WIAA) for most sports.

Prior to 1999, MUHS competed in the now defunct Wisconsin Independent Schools Athletics Association (WISAA).

The school fields teams in baseball, basketball, cross country running, downhill skiing, football, golf, ice hockey, lacrosse, rugby union, soccer, swimming, tennis, track and field, volleyball, and wrestling.

In 2010, the Hilltopper basketball team made it to the WIAA Division I state semifinals, before losing to eventual champion Arrowhead.

In 2016, The Hilltoppers made another run in the post season, eventually losing to Muskego in the WIAA Division I state semifinals.

In 2013, the MUHS lacrosse team won its second state title, with another undefeated season in Wisconsin under long-time head coach Rich Pruszynski.

[14] The team is co-ed, welcoming students from Divine Savior Holy Angels High School.

Started in 2015, the Science Olympiad program has won the state tournament 4 years in a row from 2021 to 2024 and placed 1st in the region 6 times in 2018, 2019, 2020, and 2022–24.