She attended Syracuse University, graduating with a Bachelor of Science degree in electrical engineering in 1998.

[5] She later pursued a master's degree in electrical engineering and computer science from Georgia Institute of Technology.

[5] Spio served in the U.S. Air force for 6 years as a wideband and satellite communications technician.

[7] Spio is also a writer and is the author of It's Not Rocket Science: 7 Game-Changing Traits for Uncommon Success, a self-help book published in 2015,[8] and A Song for Carmine, a love-romance novel.

[10] Mary Spio was also a keynote speaker at the Women in Tech Global Conference in 2020, where she shared insights on virtual reality and its future applications.