Matshidiso Masebe (born 29 September 2003) is a South African soccer player who plays as a goalkeeper for SAFA Women's League club UJ Ladies and the South Africa women's national team.

Masebe was named the goalkeeper of the season at the SAFA Women's League awards in 2024.

At the 2023 Women's Varsity Football she led the university to a second-place finish and was awarded the goalkeeper of the tournament with Siphesihle Dlamini from the University of the Western Cape.

[2] She ended the 2024 SAFA Women's League with 14 clean sheets from 30 appearances and was named the goalkeeper of the season.

[4][5] She made her debut in a 8-1 win over Seychelles coming in as a substitute for Jessica Williams in the 72th minute.