Matsudaira Mitsuhisa (松平光則, August 17, 1828 – December 30, 1892) was the 9th and final daimyō of Matsumoto Domain in Shinano Province, Honshū, Japan (modern-day Nagano Prefecture) and 14th hereditary chieftain of the Toda-Matsudaira clan.

In May 1842, he was proclaimed heir, and was received in formal audience with Shōgun Tokugawa Ieyoshi the same year.

In 1861, he was appointed guard for Princess Kazunomiya for her travel through Shinano on the Nakasendō between Motoyama-juku and Shimosuwa-shuku on her way to Edo to marry the Shōgun.

The following year, troops from Matsumoto were assigned to guard the British legation in Edo at the temple of Tōzen-ji; however, one of the samurai from Matsumoto, Ito Gunbei, was a member of the terrorists who attacked the legation the same year and was forced to commit seppuku for his role in the deaths of two Royal Marines.

The same year, Matsumoto was also assigned to strengthen the guard of the defenses of Edo Bay at Uraga.