Delpierre's professional career started with Lille OSC, before a move to VfB Stuttgart in Germany where he eventually became the club captain.

The following season, during which he missed only one game through illness, the French defender formed an almost impenetrable barrier alongside Fernando Meira at the heart of the Stuttgart defence.

Matthieu has also been a vital cog in Stuttgart's success in the 2006–07 DFB-Pokal, in which the team conceded only three goals before losing the final to Nürnberg.

[5] In April 2012, it was announced that Delpierre would join Hoffenheim on a free transfer at the end of the season, on a two-year contract.

[11] On 26 April 2016, Delpierre announced his retirement from professional football at the age of 35, having completed 17 seasons since his debut with Lille in 1999.