110 years prior to the series, Mayuri was an inmate in the Maggots Nest, the only one dangerous enough to be chained behind bars for his utter lack of regard for life when it comes to experimentation.

[2] Uryu is initially too weak to fight Mayuri until the Soul Reaper revealed that he experimented on Soken Ishida which his opponent unlocking his full Quincy powers prior to escaping.

[5] After defeating his controlled Zanpakuto while his opponent uses Nemu's body to reconstitute himself, Mayuri felt disappointed by the Arrancar's claims to have become a perfect being.

Soon after, Mayuri investigates Szayelaporro's laboratory to take what he can for his research before healing Uryū and Renji, followed by sending Ichigo Kurosaki and Unohana through the Garganta to the Fake Karakura Town.

While explaining to Byakuya that he is more interested in seeing what specimens Hueco Mundo offers to him, Mayuri notes that he does have faith in Ichigo's ability to end the war.

Once the fight against Aizen is settled, Mayuri attempts to seal off the Fake Karakura Town with their allies still inside but is stopped by his subordinates.

Mayuri is further livid during the Wandereich's attack on the Soul Society when his fellow captains' lost their Bankai as a result of deciding to act on their own before he could completed his analysis on the enemy.

When Toshiro Hitsugaya appears as Giselle's zombie slave, Mayuri takes on the fellow Soul Reaper captain with the intent to test some of his serums on the youth.

After having Giselle killed, while restoring Hitsugaya and Rangiku to normal, Mayuri takes Rose and Kensei to aid Byakuya in his fight against PePe Waccabrada.

After Pernida defeats Kenpachi, deeming the mysterious Wandenreich to be creepy for even him, Mayuri finds himself in a battle for his life after Perinda reveals himself to be the left arm of the Soul King.

In Bleach: Fade to Black his lab is attacked and he is driven insane, with Nemu, the rest of Squad 12 and Kenpachi trying to get him under control before they're engulfed by a white liquid from one of the machines in his laboratory.

Carlo Santos of Anime News Network said that there was "a certain amount of ridiculousness" in watching Szayelapporo Granz and Mayuri try to get the upper hand on each other during their fight.