Medford is a hamlet and census-designated place (CDP) in the Town of Brookhaven in Suffolk County, on Long Island, in New York, United States.

[2] In 1907 the LIRR established the Medford Prosperity Farm (officially called Experimental Station #2) on 80 acres (320,000 m2) to show that crops could be raised in the Pine Barrens.

As the car drove across a trail between Medford and Wading River, it got stuck in the mud and Roosevelt was said to take a "flying leap" to get out.

Eagle Estates was built along Horse Block Road east of NY 112 in 1963, although it was planned as far back as the 1930s.

The Long Island Expressway was built through Medford in 1970, with interchanges at New York State Route 112 and Horseblock Road, the latter of which is close to an older interchange with Horseblock Road and Long Island Avenue, and was not completed until 1999.

According to the United States Census Bureau, the CDP has a total area of 10.8 square miles (28.0 km2), all land.

Suffolk County Transit provides bus service (route 55 from Patchogue LIRR station to Port Jefferson).