Operated by Yarra Trams, the route is coloured turqoise green and extends from West Preston to Victoria Harbour over 13.4-kilometre (8.3 mi) of double track via St Georges Road, Fitzroy and Collins Street.

The electrification of the South Melbourne cable tram line on 25 July 1937 enabled through-running on Collins Street.

However, in August 2000, the West Preston to St Kilda - Fitzroy Street recommenced and was allocated route 112, which operated on weekdays.

Due to delays with available FNPTT rollingstock among other issues, the line couldn't open until the Melbourne & Metropolitan Tramways Board took over.

[1] Initially, the line to West Preston was single track north of the Miller Street/St Georges Road intersection (near Stop 34).

[8] It was extended a further 450 metres west to the corner of Collins and Bourke Streets on 26 January 2014, which is today's Victoria Harbour terminus.