Operated by Yarra Trams, the route is coloured dark green and extends from Airport West to Flinders Street station over 14.7 kilometre of double track via Niddrie, Essendon, Moonee Ponds Junction, Ascot Vale, Flemington, North Melbourne and Elizabeth Street.

The section of track between Abbotsford Street (near Stop 20) and Flemington Bridge station (Stop 22) was also originally part of the cable network as the North Melbourne line, which was converted to electric traction on 19 July 1925.

The line between Abbotsford Street and Haymarket was constructed and opened by the Melbourne & Metropolitan Tramways Board on the same day.

[2] The section between Flemington Bridge and Keilor Road (near Stop 46) was originally opened by the North Melbourne Electric Tramway & Lighting Company on 11 October 1906.

[7] The route starts at the corner of Matthews Avenue and Rodd Road, Airport West and operates via Niddrie, Essendon, Moonee Ponds Junction, Ascot Vale, Flemington, North Melbourne and Elizabeth Street to terminate at Flinders Street station.