Route 67 was first allocated to the line between Carnegie and the City (Swanston Street) on 1 November 1970, as part of a network-wide renumbering scheme.

Further changes on 1 August 1955 led to Route 67 becoming a shortworking for West Coburg services terminating in the City (William Street) rather than Domain Road.

Due to congestion during peak hours at the crossover, some trams continued north to Melbourne University.

This cable tram line was electrified in stages by the Melbourne & Metropolitan Tramways Board (MMTB).

The section of track between Brunning Street and Glen Huntly Road (Stop 43) opened on 29 August 1926.