Melrose is an unincorporated community in Alachua, Bradford, Clay, and Putnam counties in the U.S. state of Florida.

Local growers would bring their yearly harvest to Melrose, where it was loaded onto steam ships in Lake Santa Fe.

It encompasses 1,250 acres (5.1 km2), and contains dozens of historic buildings, the most well-known being the Trinity Episcopal Church.

Fishing in Lake Santa Fe became more popular with locals, as catfish, bass, and other animals could be caught.

The Melrose Public Library opened December 22, 1984 with Virginia Bird serving as the first librarian.