On 16 April 1957 two guards of kibbutz Mesilot were killed by Palestinian terrorists who came from the West Bank, then part of Jordan.

[2] The kibbutz also engages in various forms of agriculture, including livestock (milking cows and raising chickens), a fishery, an orchard, plantings of olives and dates, and other vegetables.

[3] The children of the kibbutz are educated in the regional school within the kibbutz, which also serves children from the nearby kibbutzim Reshafim, Nir David, Beit Hashita, Sde Nahum and Beit Alfa.

Typical children from grades 7 through 12 used to be educated at the Gilboa School, which was founded next to Kibbutz Beit Alfa and was run jointly by Mesilot, Reshafim, Nir David and Beit Alfa.

The club was home to a football team, which was sponsored by the local Wire Works factory and played under the name Hapoel Kvalim Mesilot.