Methods in Molecular Biology is a book series published by Humana Press (an imprint of Springer Science+Business Media) that covers molecular biology research methods and protocols.

The book series was introduced by series editor John M. Walker in 1983 and provides step-by-step instructions for carrying out experiments in a research lab.

[1] As of January 2020, more than 2000 volumes (2737 as of 15-August-2023)[2] had been published in the series.

Each protocol opens with an introductory overview and a list of the materials and reagents needed to complete the experiment.

Every protocol is followed by a detailed procedure that is supported with a notes section offering tips and "tricks of the trade" as well as troubleshooting advice.