The company under command of Captain James Y. Wilson mustered for service during the Spanish–American War in Jacksonville and took a train at 9 o'clock P.M. on May 13, 1898 to Tampa, arriving the following morning.

There they went to Fort Brooke and reorganized as Company F "Jacksonville Rifles", 1st Florida Infantry, mustering into Federal service on May 23.

[3] The unit was mustered into federal service with the First Florida which was redesignated 124th Infantry and mobilized at Camp Wheeler, Georgia.

Company F, 124th Infantry mobilized with its parent regiment on November 25, 1940 and trained at Camp Blanding, FL and then Fort Benning, GA.

The unit inherits the campaign history and honors of Company F, 2nd Battalion, 124th Infantry which served in New Guinea and Mindanao in the Southern Philippines.