On 7 June 2006, having been elected to the Chamber of Deputies, Biancofiore resigned as provincial councilor.

In the 2008 general election she was re-elected to the Chamber of Deputies, but in the constituency of Campania, despite her South Tyrolean origins.

On April 30, 2013 she was appointed Undersecretary for Equal Opportunities by Prime Minister Enrico Letta, but he removed her from office just two days later, following the controversy generated by her previous homophobic statements.

[4] On 1 August 2013, after the confirmation of the sentence against Silvio Berlusconi, her political leader, she resigned as Undersecretary together with the other PdL members of the government.

[8] On March 30, 2016, on the occasion of the municipal elections, she declared that wanted to leave the party after having received directives from Berlusconi himself, for a different candidate from the one she chose.

On 12 August 2017 she replaced Elisabetta Gardini as party coordinator in Trentino Alto Adige.