Microcrystallization

Although now superseded by modern analytical methods, microcrystallization still holds importance for compound purification and analysis using X-ray crystallography.

[3][4] This simple and rapid method allowed for the identification of major metabolites in hundreds of lichen species, contributing significantly to taxonomic research.

[6] Ultimately, the microcrystallization method had limitations, as it was unable to detect minor components or analyze complex mixtures of lichen substances.

[7][8] Despite these drawbacks, microcrystallization played a crucial role in the study of correlations between lichen chemistry, morphology, and geographic distribution.

[8] To perform microcrystallization, a small piece of lichen is extracted using acetone or other solvents, filtered, and evaporated to yield a residue.

In the GAW solvent system, lecanoric acid forms long, curved crystal clusters, although the results can be inconsistent, especially in the presence of other substances.