Mid Fife and Glenrothes is a constituency of the Scottish Parliament (Holyrood) covering part of the council area of Fife.

It is one of nine constituencies in the Mid Scotland and Fife electoral region, which elects seven additional members, in addition to the nine constituency MSPs, to produce a form of proportional representation for the region as a whole.

Created in 2011, the constituency comprises most of the previous Central Fife constituency which was abolished for the 2011 Scottish Parliament election.

The seat has been held by Jenny Gilruth of the Scottish National Party since the 2016 Scottish Parliament election.

The other eight constituencies of the Mid Scotland and Fife region are: Clackmannanshire and Dunblane, Cowdenbeath, Dunfermline, Kirkcaldy, North East Fife, Perthshire North, Perthshire South and Kinross-shire and Stirling.