It serves the western suburb of Footscray, in Melbourne, Victoria, Australia.

[4] Two dual gauge goods tracks run north of the station, linking the Port of Melbourne and other freight terminals to the rest of the state.

Since 1962, those tracks have formed part of the Melbourne – Sydney standard gauge line and, since 1995, have also formed part of the Melbourne – Adelaide standard gauge line.

Two Regional Rail Link tracks run south of the station.

CDC Melbourne operates three bus routes via Middle Footscray station, under contract to Public Transport Victoria: Kinetic Melbourne operates one bus route via Middle Footscray station, under contract to Public Transport Victoria: Transit Systems Victoria operates one bus route via Middle Footscray station, under contract to Public Transport Victoria: The bus stop is located on Buckley Street, about 100m from the station entrance on Victoria Street.