Established in at least the 18th century, it runs for about 0.25 miles (0.40 km), from Bridge Lane in the east to South Methven Street in the west, passing through roughly two-thirds of the northern third of the city centre.

Kinnoull Street is the only road that connects to it throughout its quarter-mile length.

[3] Pullar House, on Mill Street, was once used by J. Pullar and Sons dyeworks, the largest industry in Perth at one time, and has since been converted into office use for Perth and Kinross Council.

[4] The ancient Perth Lade passes beneath Mill Street on the final stretch of its 4.5 mi (7.2 km) journey east from Huntingtower and Ruthvenfield to Tay Street, where it discharges into the River Tay.

[7] The below vennels begin or end on Mill Street.