It is one of two ground-level stops on the Market–Frankford Line, as well as one of two SEPTA rapid transit stations located outside the Philadelphia city limits.

The station lies two blocks north of the line's namesake street.

Millbourne station is one of the original Market Street Elevated stations built by the Philadelphia Rapid Transit Company; the line opened for service on March 4, 1907 between 69th Street T.C.

[1][2] The station was closed on April 23, 2007 for rehabilitation as part of a multi-phase reconstruction of the entire western Market Street Elevated.

Following a successful pilot program where all trains made all stops, the skip-stop practice was discontinued on February 24, 2020.