The Millville and Glassboro Railroad was a railway company in the United States.

The company was merged into the West Jersey Railroad, a forerunner of the Pennsylvania-Reading Seashore Lines, in 1868.

George Brooke Roberts, a future president of the Pennsylvania Railroad, oversaw the construction of the line, which opened between Millville and Glassboro, New Jersey, in October 1860.

Some construction took place, but the line was never completed because of insufficient capital.

[4][5] The company opened its line between Millville and Cape May in August 1863.