The decision from North Central stemmed from the college's continually shaky financial situation, which culminated in a $4 million debt.
Without accreditation, the college would not have seen any federal loans or grants, adding to an already difficult situation of decreasing student enrollment.
Some 135 students had been planning to come back to campus when the school closed, many with only a few credits left until completion of their studies.
The college was a member of the NAIA and participated in the sports of baseball, basketball, and football.
The Milwaukee Bucks chose Milton College's gymnasium as its pre-season training camp in 1968-69.