Highway 336 serves as a short north–south connector route in northwest Minnesota, connecting Interstate 94 and U.S. 10, immediately east of the city of Moorhead.

It is almost entirely located in Glyndon Township, except for briefly passing through the city limits of Dilworth.

The roadway has always been a vital connection between Interstate 94 and U.S. 10, providing a gateway to nearby Detroit Lakes, a recreation destination area.

In 2002, a reconstruction project began that expanded the roadway to four-lanes and added off-ramps to its junction with U.S. Highway 10 near Dilworth, as well as a new overpass constructed over the railroad crossing.

In addition, the last 1/4 mile of Highway 336 at the north end was moved 350 feet (110 m) to the east to avoid demolition of a grain silo and an old farmstead which has since burned down.