Minnesota State Highway 65 (MN 65) is a highway in the east–central and northeast parts of the U.S. state of Minnesota, which starts at its split from I-35W, skipping past the downtown Minneapolis core, only to resume at the intersection with Washington Avenue (Hennepin County Road 152) at the north end of downtown Minneapolis to continue north to its northern terminus at its intersection with U.S. Highway 71 (US 71) in Littlefork near International Falls.

MN 65 serves as a north–south highway between Minneapolis, Fridley, Blaine, Cambridge, Mora, McGregor, Nashwauk, and Littlefork in east–central and northeast Minnesota.

The ramps end at an intersection with Tenth Street South, and so does the MN 65 designation, after MnDOT returned control of the section through downtown to the city in 2005.

Many Minnesota landmarks are located on Central Avenue NE including the Aveda Institute, the Heights Theater, and the National Sports Center.

Savanna Portage State Park in Aitkin County is located 11 miles (18 km) northeast of the junction of MN 65 and CR 14 in Shamrock Township near McGregor.

The expressway section of MN 65 between I-694 in Fridley to Spring Lake Park was constructed on a new alignment in 1953.

US 65 still enters Minnesota from Iowa, but ends now in the city of Albert Lea, where it has a junction with Interstate 35.

In July 2005, the eight-block-long section of MN 65 in downtown Minneapolis, between Washington Avenue and the junction of Tenth Street South with the on- and off-ramps to I-35W, was turned back to city maintenance.