Mississippi Highway 341 (MS 341) is a 44.871-mile-long (72.213 km) north–south state highway in the North Central Hills region of northern Mississippi, connecting Hohenlinden (via Atlanta and Vardaman) with MS 9 near Pontotoc.

It travels northeast through farmland for a few miles before climbing up some wooded hills, where it becomes concurrent (overlapped) with MS 340.

The highway winds its way north through these wooded hills for a couple miles, where MS 340 splits off along an unmarked dirt road (County Road 407, CR 407), and MS 341 descends into a narrow valley just long enough to pass through the community of Atlanta (where it makes a sharp left turn).

The highway travels straight through the center of downtown (via a one way pair), then neighborhoods, along Main Street before leaving Vardaman and traveling north through farmland for several more miles to cross a wooded ridge, where it passes through the community of Reid, before crossing the Skuna River and having a short concurrency with MS 32.

MS 341 lowers back down into a valley (the same one as last time) and meanders its way northeast through farmland for several miles, where it makes a sharp left in the community of Buckhorn, before coming to an end at an intersection with MS 9, just a half mile west of the Pontotoc city limits.