It travels directly through Magee, entering on Pinola Drive (also marked as MS 28), making a left on to Main Avenue South, a one-block jog (on 1st Street Southeast) just after Simpson Highway 149, then continuing out of town on 1st Avenue Northeast.

[5][6] A paving project from the end of the existing pavement in Magee to 5.064 miles (8.150 km) at Martinville began in 1955, and the MS 541 designation was first used in that year.

[7] Around a year later, MS 541 was extended southwards along a gravel road to U.S. Route 84 near Prentiss.

[8][9] The paving project was finished by 1958, and the route was extended northwards to MS 13 northeast of Puckett.

[14][15] The county-maintained section in Jefferson Davis County was removed from the state highway system by 1996.