It operates a 53-mile (85-km) long railroad in the St. Louis suburbs between Vigus in Union and Rock Island Junction in Overland.
The rail line was once part of the mainline of the Chicago, Rock Island and Pacific Railroad.
When the Rock Island was liquidated in 1980, the line was purchased by the Southern Pacific Railroad's subsidiary the St. Louis Southwestern Railway, known as the Cotton Belt.
The Missouri Eastern assumed from the Central Midland the lease of 8.65 miles (13.92 km) of track between Vigus and Rock Island Junction.
When it acquired the lease, the Missouri Eastern assumed Central Midland's obligations as a common carrier.