Its northern terminus is at U.S. Route 54 midway between Nevada and Deerfield.

It intersects U.S. Route 160 in Barton County west of Lamar.

In Joplin, the highway is known as Main Street and passes through the old historic downtown area.

The road serves as the western terminus of Route 90 just before reaching Southwest City.

South of Southwest City, the highway runs less than a mile before reaching the corner of Missouri, Arkansas, and Oklahoma becoming the concurrency of Arkansas Highway 43 and OK-20.