She is known for her roles as Haley Graham in the 2006 film Stick It; as Officer Andy McNally on the ABC and Global Television Network series Rookie Blue (2010–2015), for which she was nominated for a Canadian Screen Award in 2016;[1] and as FBI Special Agent Maggie Bell in the Dick Wolf–produced CBS procedural FBI, a series she has starred in since 2018.

[6] In 2000, Peregrym made her acting debut in an episode of Dark Angel, shot in Vancouver, British Columbia.

Peregrym then played the role of an illusion-generating Candice Wilmer on the television series Heroes, who was billed by production staff in promotional materials as "a female temptress".

[11] The series aired in North America, simultaneously on the Global Television Network in Canada and on ABC in the United States.

[16] In 2016, Peregrym was nominated for a Canadian Screen Award for her role in Rookie Blue,[1] but ultimately lost to Orphan Black's Tatiana Maslany.

[17] In 2017, Peregrym joined the cast of Van Helsing in its second season as Scarlett Harker, a role that she would play on and off for 14 episodes until the show's end in 2021.

[23] In 2006, Peregrym joined forces with TOMS,[24] a shoe company which also donates safe footwear for communities in South America.