She is best known for portraying the perky, squeaky-voiced blonde on the American television series Hee Haw for 19 years, often appearing as Junior Samples's assistant during used-car comedy segments.

Rowe and other "honeys" on the series were notable for performing in cleavage-inducing halter tops, hot pants, and country-style minidresses.

Rowe's 1980s television appearances include several episodes of Fantasy Island and The Love Boat and portraying a country music singer on Airwolf.

Her other film credits include The Hitchhikers (1972), Loose Shoes (1980), The Man with Bogart's Face (1980), National Lampoon's Class Reunion (1982), and Meatballs Part II (1984), and the television movie SST: Death Flight (1977).

[5] Austin, Texas rock band Young Heart Attack wrote a song called "Misty Rowe".