The various procedures used to perform moist heat sterilization process cause destruction of micro-organisms by denaturation of macromolecules.
[citation needed] Moist heat causes destruction of micro-organisms by denaturation of macromolecules, primarily proteins.
[citation needed] A more effective method is Tyndallization, which uses three successive steam treatments to achieve sterilization over the course of three days.
[citation needed] A more commonly used method when extended heat is not a concern is to use an autoclave or pressure cooker.
[3] Flash techniques generally run for the minimum time, temperature, or pressure, and may sacrifice some safeguards, such as the abilities to validate with biological indicators or prevent contamination.