The various procedures used to perform moist heat sterilization process cause destruction of micro-organisms by denaturation of macromolecules.
[citation needed] Moist heat causes destruction of micro-organisms by denaturation of macromolecules, primarily proteins.
[citation needed] A more effective method is Tyndallization, which uses three successive steam treatments to achieve sterilization over the course of three days.
This elevated temperature and pressure is sufficient to sterilize samples of any commonly encountered microbes or spores.
[3] Flash techniques generally run for the minimum time, temperature, or pressure, and may sacrifice some safeguards, such as the abilities to validate with biological indicators or prevent contamination.