Moon Woo-jin was born on February 19, 2009 in Incheon, South Korea.

In 2016, he made his first public appearance in Jeju Air's Space Museum CF then made his acting debut in 2016 with his special appearance on SBS TV series Our Gap-soon.

[2][3] Moon is affiliated to T-One Entertainment artist management company.

[4] Moon started to gain more public recognition for his acting as the young male lead in 2018 drama What's Wrong with Secretary Kim.

[6] In 2025, he performed his first villain role in his acting career through the Disney+ original drama Unmasked, where he portrayed a brutal serial killer without blood or tears.