The fight for the vacant world title was scheduled for 20 November 2009, and was held at the Wembley Indoor Arena in Johannesburg, South Africa.
[5] The IBF flyweight world title fight between Mthalane and Tete was scheduled for 1 September 2010, at the Carnival City in Brakpan, South Africa.
[6] Mthalane made his second IBF title defense against Johnriel Casimero on 26 March 2011 at the Nasrec Indoor Arena in Johannesburg, South Africa.
[8] On 1 June 2012, the IBF ordered Mthalane to defend his title against Ricardo Nunez, giving the two camps a 30-day negotiation period to come to terms.
[11] 18 months after his last fight, Mthalane was scheduled to face Jether Oliva for the vacant IBO flyweight title on 15 March 2014.
[13] Mthalane made his first IBO flyweight title defense against Odilon Zaleta on 26 October 2014 at the Olive Convention Centre in Durban, South Africa.
[14] Mthalane was scheduled to defend his IBO flyweight title for the second time against Renz Rosia on 15 December 2015, at the Olive Convention Centre in Durban, South Africa.
He faced most difficulty in the sixth round, after being knocked down by Waseem, but managed to recover sufficiently to edge out the bout in his favor.
[25] Mthalane made his first IBF title defense of his second reign against Masahiro Sakamoto on 31 December 2018 at the Wynn Palace in Macau.
[28] Mthalane made his second title defense against Masayuki Kuroda, who was at the time ranked as the ninth best flyweight in the world by The Ring, on 13 May 2019 at the Korakuen Hall in Tokyo, Japan.
[33] Mthalane was scheduled to make his fourth title defense against Sunny Edwards on 30 April 2021, 16 months from his last fight, at the York Hall in London, England.