Moses Defends Jethro's Daughters is an oil on canvas painting attributed to Italian artist Rosso Fiorentino, created c. 1523–1524, now held in the Uffizi in Florence, which acquired it in 1632.

It was first connected to the reference in Vasari by Gaetano Milanesi[4] It is unclear if the original work was sent to France or, as Antonio Natali theorises, this is a faithful copy.

Probably it is Moses himself, again, who would approach Sephora (with her breasts uncovered and the wet-effect dress that adheres to her skin) announcing the expulsion of the shepherds, while other women run away ignoring what has happened.

The scene is essentially tripartite on three levels, but the complexity of the articulation binds the plans with continuous references, subverting the traditional Renaissance balances.

Complementary colors are often juxtaposed and the geometric shapes used in the pictorial surfaces are varied: this contributes to canceling the sense of spatial depth and plasticity of the volumes, in a decidedly anticlassical direction.