Mottisfont Bats is a 196.7-hectare (486-acre) biological Site of Special Scientific Interest west of Winchester in Hampshire.

[3] The site consists of woods around Mottisfont, which supports a population of the barbastelle bat which is considered by Natural England to be of national importance.

The site is used by the barbastelles for breeding, roosting, commuting and feeding.

It is the only known maternity roost in Hampshire and one of only six known sites in the United Kingdom as of 2002.

Eight other bat species have been recorded at Mottisfont: whiskered, brown long-eared, common and soprano pipistrelles, serotine, noctule, Daubenton's and Natterer's.