[5] It focuses on the misadventures of two friends, Motu and Patlu, landing in trouble and comical situations, later being rescued only by luck.

[7] It is a peaceful yet glamorous small town, simultaneously noble and squalid, adjoined by a river with a few shops, a large market area, a bus depot, a cluster of single-storeyed houses, temples, and a few recreational places.

It is not clearly mentioned where the town is exactly located, but most of the vehicles carry license plate numbers starting with "MH", which stands for the state of Maharashtra.

Some Indian cities of monuments they have visited include Taj Mahal (Agra), Gateway Of India (Mumbai), Victoria Memorial (Kolkata), Qutub Minar (New Delhi), Hawa Mahal (Jaipur), Tea Plantation (Assam), Backwater (Kerala), Charminar (Hyderabad), Bandra-Worli Sea Link (Mumbai), Rock Garden (Chandigarh), Elephanta Caves (Mumbai), Bekal Fort (Kerala), Red Fort (New Delhi) and Howrah Bridge (Kolkata) and many more.

Motu is mainly the black sheep of the duo, unintentionally creating problems due to his incompetency while Patlu is the smart one who always tries to stop him.

The theme song of the series, "Motu Aur Patlu Ki Jodi", is written by Gulzar and sung by Bollywood singer Sukhwinder Singh in Hindi.

[14] Numerous mobile video games based on the series have been released by Indian software companies such as Nazara Technologies and Tangiapps.