[2][3] The Museumsinsel station has a single central platform at the eastern end of Unter den Linden, between Zeughaus and Berlin Palace.

Part of the station is located just south of the Schlossbrücke under the Spree, at a depth of 16 meters below the upper edge of the street.

Both ends are equipped with escalators, and near the east entrance is an elevator leading from the surface directly to the platform.

The design by Max Dudler was inspired by a stage design by Karl Friedrich Schinkel for the opera The Magic Flute (Die Zauberflöte) from 1816 and features a starry sky on a dark blue barrel vault with points of light over the tracks.

A cooling liquid consisting of −37 °C cold calcium chloride solution was pumped into these holes, freezing the soil so that the future platform area could be broken up.

All equipment for the operation, such as power supply, telecommunication and air conditioning systems have been installed in the underground station building.