[1] The hospital is located in the neighborhood called Mutolere, a suburb of Kisoro Town, approximately 6 kilometres (3.7 mi), by road, northeast of Kisoro Town, where the district headquarters are located.

[2] This location, lies in extreme southwestern Uganda, close to the International borders with the Democratic Republic of the Congo and with the Republic of Rwanda.

Mutolere Hospital lies approximately 71 kilometres (44 mi), west of Kabale Regional Referral Hospital, in the city of Kabale, the nearest large urban centre.

It is accredited to the Uganda Catholic Medical Bureau, and it is administered by the Little Sisters of Saint Francis, a religious congregation.

One such partner is the non-profit organization, Sustain for Life which, in collaboration with Mutolere Hospital, provides "healthcare, preventative medicine and education" to the Batwa communities in Kisoro District, Uganda.