He was elevated to the rank of Cossack colonel in 1616, and he was active in Petro Konashevych's wars against Muscovy.
In late 1625 Stanisław Koniecpolski made him sign the Treaty of Kurukove in which the Cossacks renounced an independent foreign policy.
This led him to conflicts with the Zaporozhian Cossacks who had opposed the treaty.
In 2013, with the support of the museum "muzei Hetmanstva" the "Hetman Petro Doroshenko fund" was created.
The Fund carries out research activity about the Hetmans of Ukraine: Myhailo and Petro Doroshenko, shares the information about them, researches genealogy of Doroshenko.