Specimens found along the coast are generally darker in coloration and are considered to be part of the subspecies Myotis evotis pacificus.

It has been known to occur in semiarid shrublands, shortgrass prairie, and subalpine forests, with habitats ranging from sea level to 2,830 meters (9,280 ft).

[3] They roost in a variety of places, including tree cavities, rock crevices, caves, and even abandoned buildings.

They seem to prefer rock crevices, while individuals in the northern part of the range favor ponderosa and lodgepole pines.

[5] The long-eared myotis is an insectivore, whose robust molars and highly placed articular process allow it be especially good at hunting beetles.