Natalio is a district in the Itapúa Department of Paraguay.

It is located in the South-East of the country, with the town center located just 10 km from the Paraná River and the border with Argentina.

The district lies 45 km off Route 6 which connects departmental capitals Encarnación and Ciudad del Este.

The town is located in a fertile agricultural region.

Local crops are soy, corn, wheat, tung, sorghum, and cotton.