[1] Mather was assistant to George Mortimer (died 1688) at Harberton,[1][2] Devonshire (a Parliamentary sequestered vicarage), and succeeded him there in 1655.
In 1656 he was presented by the Lord Protector Oliver Cromwell to the sequestered vicarage of Barnstaple, Devonshire, in which the vicar, Martin Blake (1593–1673), B.D., was reinstated at the Restoration.
In 1688, the year of the Glorious Revolution, he left Ireland, and became pastor of the independent church in Paved Alley, Lime Street, London, vacant by the death of John Collins (1632?–1687).
[4] Mather joined the "Happy Union" of 1691, but was a leader in its disruption owing to the alleged heresies of Daniel Williams (1643?–1716), D.D.
[6] He published:[6] Posthumous were:[6] On 2 August 1655 Nathaniel Mather married Mary Benn, the daughter of the Revd.