The act also forbade foreign war vessels to outfit in American waters and set a three-mile territorial limit at sea.

The Neutrality Act of 1794 was used in the trials of Aaron Burr, William S. Smith and Etienne Guinet, who, with Frenchman Jean Baptist LeMaitre, were convicted of outfitting an armed ship to take part in France's war against Great Britain.

[4] On March 13, 1794, the Vice President, John Adams, broke a tie in the Senate in favor of the bill that would become the Neutrality Act of 1794.

[14] In 1981, nine men involved in Operation Red Dog were each sentenced to three years in prison pursuant to the successor statute of the Neutrality Act of 1794; they had planned to overthrow the government of Dominica.

In May 2016 four United States residents were convicted of violating the successor statute of the Neutrality Act of 1794 for their role in the 2014 Gambian coup d'état attempt.