Route 540 is a 54.8-kilometre (34.1 mi) long provincial highway located in western New Brunswick, Canada.

Route 540 begins in Graham Corner, near Eel River Lake, and travels north through rural woodlands in northern York County, passing through the community of Maxwell, and by Belle Lake.

The highway continues north, into Carleton County and the community of Kirkland, where the woodlands give way to farmland.

Past Route 95, the highway continues through several small communities and rural farmland, before turning east near Oakville.

The highway comes to an end in Lindsay at an intersection with Route 550.