Sir Nicholas Attygalle KBE FRCS FRCOG (14 July 1894 – 27 March 1970) was a Ceylonese academic, surgeon and a Senator.

Attygalle was the first Ceylonese to obtain MRCOG (Gr Britain) after which he spent time in Vienna to follow a course in Gynaecological Pathology and Physiology where he also studied operative techniques in Gynecology.

Soon after he was enrolled as a member of the Austrian Medical Association and succeeded Dr. Lucian De Zilva as the Gynecologist of the General Hospital in 1935.

Holding the post until 1953, Departments of Bacteriology, Biochemistry, Paediatrics, Parasitology and Pharmacology were established in the Colombo Medical Faculty and postgraduate examinations in Medicine (MD, MS and MOG) also commenced during this period.

In 1954 he became the first Ceylonese Vice-Chancellor of University of Ceylon, succeeding Sir Ivor Jennings, a post he head till his retirement in 1967.

Attygalle served as president of the Sri Lanka Medical Association and chairman of the National Science Council.