Bertha Nicoline Tuxen (14 November 1847 – 5 April 1931) was a Danish painter of still lifes, flowers, and portraits.

In 1891 she was awarded the Neuhausen Prize (De Neuhausenske Præmier) and in 1893 with a scholarship from the Royal Academy.

Nicoline Tuxen was regularly represented at the prestigious Charlottenborg Spring Exhibition (Forårsudstilling) in Copenhagen from the middle of the 1880s.

Their works bear the unmistakable stamp of the Danish Golden Age of painting.

Nicoline Tuxen remained unmarried, she died in Frederiksberg at the age of 83 and was buried at the Holmens Cemetery in Copenhagen.